Psichecktm-2 vector

Author: tnme

August undefined, 2024

WebApr 9, 2024 · 作者签名：产m堤日期：2口出年‘月侈日学位论文版权使用授权书学位论文作者完全了解华中师范大学有关保留、使用学位论文的规定，即：研究生在校攻读学位期间论文工作的知识产权单位属华中师范大学。 ... －CHECK2 recombinant expressive vector carrying ... WebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target …

How to Convert Matrix to Vector in R (With Examples) - Statology

WebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. Shop Promega psiCHECK™ Vectors at … WebpsiCHECK-2 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … goan chicken curry

Psichecktm 2 Vector Promega Bioz

http://www.youbio.cn/product/vt1400 Webgastric cDNA and inserted into the psiCHECKTM-2 vector (Promega, Madison, WI, USA). The mutant-type SOX4 3'-UTR was generated using QuikChange Site-Directed Mutagenesis kit (Stratagene, La Jolla, CA, USA) as per the manufac - turer's protocol, and then inserted into the psiCHECKTM-2 vector. For the dual-luciferase reporter assay, SGC-7901 WebFeb 8, 2024 · PsiCHECKTM‐2 vector without inserted gene was used as negative control (NC) plasmid. The inserted sequences were listed in Table. Table.1. 1. Following the procedures provided by Ribobio and Promega, A7r5 cells were co‐transfected with PsiCHECKTM‐2 vector (WT/MUT/NC) and oligonucleotides (miR‐137 mimic or mimic … bonds secure

miR-138 inhibits gastric cancer growth by suppressing SOX4

Long noncoding RNA SNHG14 promotes hepatocellular …

WebSep 4, 2024 · I had A vector A=[1 0 0 2 3 2 3 5 0 0 0 0 1 2 1 0 0 0 0 3 4 0 0 0 0 0 0 1 2 3 2 0 0 0 0 2 0 0] like this. Now I want a vector B having average of corresponding non ... WebJun 17, 2024 · Example 2: Convert Matrix to Vector (sorted by rows) Using c() function. The following code shows how to convert a matrix to a vector (sorted by rows) using the c() function: #convert matrix to vector (sorted by rows) new_vector <- c(t(my_matrix)) #display vector new_vector [1] 1 6 11 16 2 7 12 17 3 8 13 18 4 9 14 19 5 10 15 20 Example 3 ... bonds savings fifteenth anniversaryWebApr 15, 2024 · Fig. 2: Néel vector control by cooling in opposite magnetic fields. a Optical image and its MCD image at 280 K of the front ab plane in a 500 μm-thick crystal. bonds secured tangible asset

"WebHEK293T cells were transfected with psiCHECKTM-2-Wt-IGF2BP2 or psiCHECKTM-2-mut-IGF2BP2, together with miR-138 mimics, inhibitor or the negative control (GenePharma, China) by Lipofectamine 2000 (Invitrogen, USA). Forty- eight hours later, relative luciferase activities were assessed using the Dual-Luciferase Reporter Assay System (Promega, USA). " - Psichecktm-2 vector

Psichecktm-2 vector

LincRNA-p21 Inhibits Cell Viability and Promotes Cell ... - Hindawi

WebpsiCHECKTM-2-vector and 50nM of the miR-876-5p or mimic-NC using ExFect®2000 Transfection Reagent (Vazyme Biotech Co., Ltd). Cell lysates were prepared using Passive Lysis Buffer (Promega) 48h after transfec-tion, and luciferase activity was measured using the Dual-Luciferase Reporter Assay (Promega). Experiments were repeated three times. WebMar 10, 2024 · In order to do a vector search, our input text needs to be converted to an embedding as well. The second parameter to this function “k” is the number of objects we want to return that is closest to our input. We want to find the closest vectors to our input vector so we chain the .with_near_vector() function.

Did you know?

WebHere we demonstrate the use of the psiCHECK™-2 Vector as a biosensor for miRNA-21 (miR-21) in different cell lines and as a tool to test an miRNA target. Generating an miRNA … WebPsicheck Tm 2 Dual Luciferase Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, …

Webthe PsicheckTM-2 vector to generate PsicheckTM-2-PPAR γ-WT.ThemiR-377bindingsiteinPPAR wasthenmutated,andthemutantfragmentwas 2-PPARγ-MT.Forty … Web2.2 测序及序列对比分析含有hsa-miR-181b结合位点及缺失hsa-miR-181b结合位点的荧光素酶报告载体psiCHECK2-TNF和psiCHECK2-TNF-mut测序结果(图3、图4)。可见TNF的目标序列和突变序列均成功地克隆入双萤光报告载体psiCHECK2，可用于后续实验。

http://www.biovector.net/product/44170.html WebShuffle T7 Express E.coli Strain大肠杆菌表达菌株及感受态细胞. 价格： ¥0. 货号： 3544763. 产地：北京. 点击询问我要采购. 竭诚为您服务！.

WebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference …

WebDec 8, 2016 · The PCR products were digested with XhoI and NotI and were then ligated into the reporter vector psiCHECKTM-2 (TWIST1 3’-UTR-8bp-deletion). The overexpression construct pFLAG-TWIST1 (pTWIST1) containing the TWIST1 protein coding region without the 3’UTR and pFLAG-CMV (empty vector, pEV) was a gift from Prof. Muh-Hwa Yang at … bond ssaWebMyocyte enhancer factor (MEF2) is a family of transcrip- tion factors that binds to an A/T-rich DNA element in the promoter regions of muscle-speciﬁcgenes(12). MEF2 exerts a diversity of function during myogenic differentiation and is involved in the control of various aspects of muscle ﬁber properties (13). goan christianWebBy cloning the 3’UTRs of collagen IV subunits into the psiCHECKTM-2 vector, we found that seven of the eight sites in the Col4a3–Col4a6 gene complementary to miR-29 were significantly repressed by miR-29a, b (except for the 7774–7781 of Col4a3 gene). The inhibitory efficiency of miR-29a, b on these seven sites was between 27% and 57%. bonds secure columbia mdWebAug 13, 2024 · The 3′UTR of CREBL2 and PCBP2 were sub-cloned into the psiCHECK-2 vector (Promega, USA) downstream of the Renilla luciferase reporter gene. Flanking sequence on each side was synthetized with a short extension containing cleavage sites for XhoI (CTCGAG) and NotI (GCGGCCGC), and the primer sequences are shown in Table … goan chicken gravyWebThe 3 -UTR sequence of PPARγwas cloned and inserted into the PsicheckTM-2 vector to generate PsicheckTM- 2-PPARγ-WT.ThemiR-377bindingsiteinPPAR wasthenmutated,andthemutantfragmentwas 2-PPARγ-MT.Forty-eighthoursaftertheaforemen- tioned constructs were transfected, the luciferase activities … bonds section yahoo financeWebエムエスアイコンピュータージャパンは4月13日、プロeスポーツプレイヤー向けをうたったハイエンド構成の16型ゲーミングノートPC「Vector GP68HX 12V ... bonds seamless undershortsWebMar 26, 2024 · The pGL3.0 luciferase reporter vector (E1910; Promega, Madison, WI) and psiCHECKTM-2 vector (C8021, Promega) were used for the dual-luciferase reporter assay to verify the binding sites in sequences under study for Sp1 and miR-375, respectively. bonds seamless hi top gee